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modified langendorff perfusion apparatus  (Radnoti LLC)

 
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    Radnoti LLC modified langendorff perfusion apparatus
    Modified Langendorff Perfusion Apparatus, supplied by Radnoti LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/modified+langendorff+perfusion+apparatus/pmc02234135-151-7-10?v=Radnoti+LLC
    Average 90 stars, based on 1 article reviews
    modified langendorff perfusion apparatus - by Bioz Stars, 2026-07
    90/100 stars

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    a–b, Immunofluorescent staining of ( a ) control right atrium and ( b ) gene painted SAN showing DAPI (blue) and HCN4 (red) to identify SAN, and Myc epitope tag (green) for DN-MCU-Myc expression. Scale bars are 200 μm. c–d, Example ex vivo ECG tracings recorded under basal (left) and ISO stimulated (right) conditions from gene painted hearts. Scale bar is 100 ms. e, Summary dose response data for ex vivo heart rate response to ISO. f , Percentage rate increases over baseline after ISO in gene-painted isolated, <t>Langendorff-perfused</t> hearts (data calculated from Fig. 2e). ***p<0.001, unpaired t test, n=22–24/group in panel e. Error bars indicate SEM.
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    a–b, Immunofluorescent staining of ( a ) control right atrium and ( b ) gene painted SAN showing DAPI (blue) and HCN4 (red) to identify SAN, and Myc epitope tag (green) for DN-MCU-Myc expression. Scale bars are 200 μm. c–d, Example ex vivo ECG tracings recorded under basal (left) and ISO stimulated (right) conditions from gene painted hearts. Scale bar is 100 ms. e, Summary dose response data for ex vivo heart rate response to ISO. f , Percentage rate increases over baseline after ISO in gene-painted isolated, <t>Langendorff-perfused</t> hearts (data calculated from Fig. 2e). ***p<0.001, unpaired t test, n=22–24/group in panel e. Error bars indicate SEM.
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    modified langendorff perfusion apparatus  (Radnoti LLC)
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    a–b, Immunofluorescent staining of ( a ) control right atrium and ( b ) gene painted SAN showing DAPI (blue) and HCN4 (red) to identify SAN, and Myc epitope tag (green) for DN-MCU-Myc expression. Scale bars are 200 μm. c–d, Example ex vivo ECG tracings recorded under basal (left) and ISO stimulated (right) conditions from gene painted hearts. Scale bar is 100 ms. e, Summary dose response data for ex vivo heart rate response to ISO. f , Percentage rate increases over baseline after ISO in gene-painted isolated, <t>Langendorff-perfused</t> hearts (data calculated from Fig. 2e). ***p<0.001, unpaired t test, n=22–24/group in panel e. Error bars indicate SEM.
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    modified langendorff heart perfusion apparatus  (Radnoti LLC)
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    a–b, Immunofluorescent staining of ( a ) control right atrium and ( b ) gene painted SAN showing DAPI (blue) and HCN4 (red) to identify SAN, and Myc epitope tag (green) for DN-MCU-Myc expression. Scale bars are 200 μm. c–d, Example ex vivo ECG tracings recorded under basal (left) and ISO stimulated (right) conditions from gene painted hearts. Scale bar is 100 ms. e, Summary dose response data for ex vivo heart rate response to ISO. f , Percentage rate increases over baseline after ISO in gene-painted isolated, <t>Langendorff-perfused</t> hearts (data calculated from Fig. 2e). ***p<0.001, unpaired t test, n=22–24/group in panel e. Error bars indicate SEM.
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    a–b, Immunofluorescent staining of ( a ) control right atrium and ( b ) gene painted SAN showing DAPI (blue) and HCN4 (red) to identify SAN, and Myc epitope tag (green) for DN-MCU-Myc expression. Scale bars are 200 μm. c–d, Example ex vivo ECG tracings recorded under basal (left) and ISO stimulated (right) conditions from gene painted hearts. Scale bar is 100 ms. e, Summary dose response data for ex vivo heart rate response to ISO. f , Percentage rate increases over baseline after ISO in gene-painted isolated, Langendorff-perfused hearts (data calculated from Fig. 2e). ***p<0.001, unpaired t test, n=22–24/group in panel e. Error bars indicate SEM.

    Journal: Nature communications

    Article Title: The mitochondrial uniporter controls fight or flight heart rate increases

    doi: 10.1038/ncomms7081

    Figure Lengend Snippet: a–b, Immunofluorescent staining of ( a ) control right atrium and ( b ) gene painted SAN showing DAPI (blue) and HCN4 (red) to identify SAN, and Myc epitope tag (green) for DN-MCU-Myc expression. Scale bars are 200 μm. c–d, Example ex vivo ECG tracings recorded under basal (left) and ISO stimulated (right) conditions from gene painted hearts. Scale bar is 100 ms. e, Summary dose response data for ex vivo heart rate response to ISO. f , Percentage rate increases over baseline after ISO in gene-painted isolated, Langendorff-perfused hearts (data calculated from Fig. 2e). ***p<0.001, unpaired t test, n=22–24/group in panel e. Error bars indicate SEM.

    Article Snippet: Briefly, excised hearts from 7–9 week female and male mice were rapidly mounted on a modified Langendorff apparatus (HSE-HA perfusion systems, Harvard Apparatus, Holliston, Mass) for retrograde aortic perfusion at a constant pressure of 80 mm Hg with oxygenated (95% O 2 , 5% CO 2 ) Krebs-Henseleit solution consisting of (mM) 25.0 NaHCO 3 , 118.5 NaCl, 4.0 KCl, 1.2 MgSO 4 , 1.2 NaH 2 PO 4 , 1.5 CaCl 2 , and 11.2 glucose, with pH equilibrated to 7.4.

    Techniques: Staining, Expressing, Ex Vivo, Isolation